| 1. | Third , quality policy : the strict management , the positive control , provide the high quality product and the service for the customer
三质量方针：严格管理全面控制，为客户提供优质的产品和服务。 |
| 2. | Dimensional uniformity of closed - die forgings results in positive control of critical wall thickness , eliminating deficiencies caused by shifted cores in castings
闭模锻造的尺寸一致性造成关键壁厚的完全控制，避免了铸造工艺中铁心移位造成的缺陷。 |
| 3. | Note : “ + ” denotes bacterial growth and “ - ” denotes no bacterial growth . the positive control had bacterial growth and the negative control had no bacterial growth
注： “ + ”表示有菌生长， “ - ”表示无菌生长。阳性对照有细菌生长，阴性对照无菌生长。 |
| 4. | The primers are designed in sites of the number 2 and 3 exon of hbrp gene . the product of rt - pcr by using human b - actin as primer is looked on as the positive control
Lthionesepharose4b纯化表达的gst和gst hb即融合蛋白，分别检测不同浓度的gst蛋白及gsp hb地融合蛋白对pkc活性的 |
| 5. | Stem : the tee - head disc - stem connection prevents lateral strain on the stem for smooth , easy operation . accurately cut threads engage the yoke sleeve for positive control of disc position
阀杆： t形阀瓣-阀杆连接防止施加在阀杆侧面的应力，确保操作平稳顺畅容易。轭架套管准确的螺纹连接有助于阀瓣位置的调整控制。 |
| 6. | Methods 1 ) statistic methods including factorial experiment was carried out to optimize the major conditions for sample management , and the feasible negative and positive control for fcm analysis of cd62p expression were check out
方法1采用浓度梯度法优化gprp浓度条件，采用析因设计优化凝血酶浓度和37孵育时间条件，寻找最佳阴、阳性对照。 |
| 7. | It is efficient for flow cytometric analysis when fresh platelet riched plasma ( fprp ) was set as negative control , thrombin actived fprp and liquid nitrogen treated fprp were set as positive control respectivelv
除了fcm常用的同型对照外，文中还将fpr 、凝血酶激活fprp和液氮处理fprp作为血小板标准阴性、阳性对照，直接应用于fcm分析，且效果良好。 |
| 8. | 5 . prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate , meantime , add p2 , low homologic probes and probe buffer in the same concentration as positive control , blank control and negative control respectively . spot microarray as designed by the spotting machine
5 .寡核昔酸微阵列的制备以同等浓度的反义链引物咫作为阳性对照，以探针缓冲液作为空白对照，以无关探针作为阴性对照，与制备好的16条寡核昔酸探针，同时加人384孔板。 |
| 9. | Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank , a universal primer set l15774 / hsf21 was used as positive control of the template quality , at the meantime , two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ) , sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species . a musk deer ( moschus ) specific primer set wf / mr was designed , siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products
经过对来自genbank中的15种鹿类动物的cytob基因序列的比较，用通用引物l15774和hsf21作为模板的质量控制，设计了特异性引物df dr和cf cr来鉴定马鹿、梅花鹿、狍；设计了麝类特异性引物mf mr ，用限制性内切酶rsa酶切扩增产物来区分原麝和林麝。 |
| 10. | Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control
选取单个菌落分别点种到删平板和md平板，找出在回d上生长正常， w上生长缓慢或不生长的菌落，即阳性菌落，再以阳性菌落分别涂布zeocine含量500ug加， 1000ug砌l的ypd平板，以高浓度的抗生素筛选高拷贝的酵母工程菌，在含500ug ml高浓度抗生素平板上获得了15个转化子，取其中5个进行pcr扩增，有3个扩增产物与阳性对照相同，说明此酵母细胞中已含有s hbsag融合片段，其中之一命名为p |
